Journal: Applied and Environmental Microbiology

Article Title: Determination of Clonality and Relatedness of Vibrio cholerae Isolates by Genomic Fingerprinting, Using Long-Range Repetitive Element Sequence-Based PCR

doi: 10.1128/aem.00151-08

Figure Lengend Snippet: FIG. 1. Effect of electrophoresis buffer on banding patterns in ERIC-PCR fingerprinting, using a 1.0% agarose gel and a 0.75-mm comb. (A) 1 TAE buffer. (B) 0.5 TBE buffer. Lanes: M, size markers; 1 to 3, V. mimicus RC 217; 4 to 6, V. cholerae RC 561; 7, V. cholerae O1 El Tor N16961. Triangles between lanes show bands that are more clearly visible in gels using TBE.

Article Snippet: Electrophoresis was performed at 120 V for 2.25 h, using a Bio-Rad GT Subcell electrophoresis system (electrode distance, 30 cm) with a 15-cm by 20-cm tray.

Techniques: Electrophoresis, Agarose Gel Electrophoresis

Journal: Applied and Environmental Microbiology

Article Title: Determination of Clonality and Relatedness of Vibrio cholerae Isolates by Genomic Fingerprinting, Using Long-Range Repetitive Element Sequence-Based PCR

doi: 10.1128/aem.00151-08

Figure Lengend Snippet: FIG. 3. Variability in similarities of fingerprints. The results of 21 PCRs and electrophoresis of genomic DNA of V. cholerae O1 El Tor N16961 are shown. The dendrogram shows a tree created by UPGMA clustering based on the Pearson correlation coefficient for the range of 0.4 to 10 kb. The scale bar at the top of the dendrogram shows the Pearson correlation coefficient (%); the minimum similarity coefficient in the dendrogram is 89.66%.

Article Snippet: Electrophoresis was performed at 120 V for 2.25 h, using a Bio-Rad GT Subcell electrophoresis system (electrode distance, 30 cm) with a 15-cm by 20-cm tray.

Techniques: Electrophoresis